FastQCFastQC Report
Sun 20 Nov 2022
CO_006-RNA.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_006-RNA.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46657
Sequences flagged as poor quality0
Sequence length25-347
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACATCTGTCCAGT877318.803180658850764No Hit
GCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATTTCTTCCTCAT516411.068006944295604No Hit
GAGGAGACACCGCCCACCACCAGCAGCGACTCTGAGGAGGAACAAGAAGA39418.446749683863086No Hit
TACTTAAGCAGGTACCAAACCCAGGCCCTCCTCCCCGCTGCCCATCTCCC37818.103821505883362No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTTGCAGCTG28376.0805452558029875No Hit
CACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTACCGCAGCTGC26375.651885033328332No Hit
GCGAAGAGCAGCCCAGCTGCAGAGAAAGTTCCCGCATCTGGAGTTCAGGA17773.808646076687314No Hit
TACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGATTTTGACTACCGA15863.3992755642240176No Hit
CCTGCTGCTCGTCCAGCTTCCGAAGCCGGGTGTTGAGGTTTCCCCGAATA14293.062777289581413No Hit
GCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTTGGTGGAGGAT11842.53766851704996No Hit
GGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTCATCCTCCACCAAG9492.0339927556422404No Hit
CTGACCTTTTGCCAGGAGCCTGCCTCTTTTCCACAGAAACAACATCGATT7371.5796129198191053No Hit
TACTTAAGCAGGTACCAAACCCAGGCCCTCCTCCCCGCTGCCCATCCTCC3270.7008594637460617No Hit
GCTCCCGTTAAGGTGCATCCTTCGGATCATGCTGCCCTGGGTGGTCACAT3140.6729965492852091No Hit
GTGCGTGGTTTGCCAAGATATCGCGTGGTTTGCC3080.6601367426109694No Hit
GCAATGGTCTTTAGGTGGGAACACTGT2970.6365604303748633No Hit
GGCAAACCACGCGATATCTTGGCAAACCACGCAC2790.5979810103521443No Hit
GACCCCCATCACTCCTGCCACGCCAGCTTCGGAGAGTTCTGGGATTGTAC2610.5594015903294254No Hit
CCAACTACACGTTACTTAAGCAGGGCCTGAACAACGCCGTTGCCTTGGAT2330.49938915918297366No Hit
ACAGTGTTCCCACCTAAAGACCATTGC2160.4629530402726279No Hit
GCTCCCTTTTCTTGGGGTCTCACTCTGAGCACGACCGTGCCTCTGGCGTC2020.43294682469940204No Hit
CCTGCTGATCGTCCAGCTTCCGAAGCCGGGTGTTGAGGTTTCCCCGAATA1600.3429281779797244No Hit
CCAACTACACGTTACTTAAGCAGGTACCAAACCCAGGCCCTCCTCCCCGC1570.33649827464260457No Hit
CCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCC950.20361360567546136No Hit
AGCCACGGAACCTCCAAGAATGTTCTGTT920.1971837023383415No Hit
CCAACTACACGTTACTTAAGCAGGTACCCAAACCCAGGCCCTCCTCCCCG900.19289710011359495No Hit
CGAAGCGCAGTAAGGATGGCTAGTGTAACTAGCAAGAATACCACGAAAGC890.1907537990012217No Hit
GCCAGGAGCCTGCCTCTTTCCACAGAAACAACATCGATTTCTTCCTCATC880.1886104978888484No Hit
AGGTCAAGTTTACAACCAAGATTCACTGTGGATACAATATTTCTGTAAGA730.15646098120324925No Hit
GATGGCTGGTGCAAAGAGCGGACCCTGGACAACCAGCTGTTCTTCTTCTT730.15646098120324925No Hit
AACAGAACATTCTTGGAGGTTCCGTGGCT690.14788777675375614No Hit
GAGGAGACACCGCCCACCACCAGCAGCGACTCTGGAGGAACAAGAAGATG530.1135949589557837No Hit
TACTTAAGCAGGTACCCAAACCCAGGCCCTCCTCCCCGCTGCCCATCCTC500.10716505561866385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)